TaqMan-based real-time polymerase chain reaction assay for specific detection of bocavirus-1 in domestic cats
نویسندگان
چکیده
منابع مشابه
A New TaqMan Real-Time Polymerase Chain Reaction Assay for Quantification of Fusarium virguliforme in Soil
Mbofung, G. C. Y., Fessehaie, A., Bhattacharyya, M. K., and Leandro, L. F. S. 2011. A new TaqMan real-time polymerase chain reaction assay for quantification of Fusarium virguliforme in soil. Plant Dis. 95:1420-1426. The quantification of the soilborne pathogen Fusarium virguliforme inoculum in soil is important for epidemiological studies of soybean sudden death syndrome (SDS). Classical dilut...
متن کاملDetection of Nocardia Asteroides Complex in Clinical Isolates by Real-Time Polymerase Chain Reaction
Background and Aims: Nocardia asteroides complex is the most common cause of infectious diseases due to nocardiosis. Interspecies differentiation of Nocardia genera is essential for prognosis and timely proper treatment, as well as for epidemiological studies. Since each genus has its own antibiotic resistance, precise careful diagnosis is of prime importance. As compared to biochemical and phe...
متن کاملRapid Detection of Porcine Circovirus Type 2 by TaqMan-based Real-time Polymerase Chain Reaction Assays
PCV2 is the primary causative agent of porcine circovirus-associated disease (PCVAD). In this study, a TaqMan-based real-time polymerase chain reaction (PCR) assay targeting ORF2 gene of PCV2 was developed and their sensitivities and specificities were investigated. The results indicated that the standard curve had a wide dynamic range (102-107copies/μL) with a linear correlation (R2) of 0.997 ...
متن کاملevaluation of the performance characteristics of an in-house one step taqman real time-polymerase chain reaction assay for detection and quantification of hepatitis c virus
conclusions the in-house 1 step taqman real time rt-pcr assay showed acceptable performance characteristics. our study presents the robustness and cost-effectiveness of the method for detection and quantification of hcv rna. methods the primers and probe were selected from a highly conserved region of the hcv genome, which allowed the detection of 4 common hcv genotypes in iran. using 4 quantif...
متن کاملA Multiplex Real-Time Polymerase Chain Reaction (TaqMan) Assay for the Simultaneous Detection of Meloidogyne chitwoodi and M. fallax.
ABSTRACT This study describes a multiplex real-time polymerase chain reaction (PCR) approach for the simultaneous detection of Meloidogyne chitwoodi and M. fallax in a single assay. The approach uses three fluorogenic minor groove binding (MGB) TaqMan probes: one FAM-labeled to detect M. chitwoodi, one VIC-labeled to detect M. fallax, and one NED-labeled to detect the internal amplification con...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular and Cellular Probes
سال: 2020
ISSN: 0890-8508
DOI: 10.1016/j.mcp.2020.101647